Recombinant Human GSTT1 Protein, His-tagged

• Cat.No.: GSTT1-2223H
• Product Overview: Recombinant Human GSTT1 Protein (Phe20-Gln229) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Phe20-Gln229
• Description: The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.4 kDa
  Accurate Molecular Mass: 27 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GSTT1 glutathione S-transferase theta 1 [ Homo sapiens (human) ]
• Official Symbol: GSTT1
• Synonyms: GSTT1; glutathione S-transferase theta 1; glutathione S-transferase theta-1; GST class-theta-1; glutathione transferase T1-1
• Gene ID: 2952
• mRNA Refseq: NM_000853
• Protein Refseq: NP_000844
• MIM: 600436
• UniProt ID: P30711