Recombinant Human GSR, His-tagged

Description :	This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Protein length :	417 amino acids
Conjugation :	HIS
Molecular Weight :	54.300kDa inclusive of tags
Source :	E. coli
Biological activity :	Recombinant human GSR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Form :	Liquid
Purity :	>95% by SDS-PAGE
Storage buffer :	pH: 8.00Constituents:0.32% Tris HCl, 0.02% DTT, 10% Glycerol, 0.58% Sodium chloride
Storage :	Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Sequences of amino acids :	MGSSHHHHHH SSGLVPRGSH MGSMPKKVMWNTAV HSEFMHDHAD YGFPSCEGKF NWRVIKEKRD AYVSRLNAIY QNNLTKSHIE IIRGHAAFTS DPKPTIEVSG KKYTAPHILI ATGGMPSTPH ESQIPGASLG ITSDGFFQLE ELPGRSVIVG AGYIAVEMAG ILSALGSKTS LMIRHDKVLR SFDSMISTNC TEELENAGVE AMACRQ EPQPQGPPPA AGAVASYDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCVVLKFSQVKEV KKTLSGLEVS MVTAVPGRLP VMTMIPDVDC LLWAIGRVPN TKDLSLNKLG IQTDDKGHII VDEFQNTNVK GIYAVGDVCG KALLTPVAIA AGRKLAHRLF EYKEDSKLDY NNIPTVVFSH PPIGTVGLTE DEAIHKYGIE NVKTYSTSFT PMYHAVTKRK TKCVMKMVCANKEEKVVGIH MQGLGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR
Sequence Similarities :	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Gene Name	GSR glutathione reductase [ Homo sapiens ]
Official Symbol	GSR
Synonyms	GSR; glutathione reductase; glutathione reductase, mitochondrial;
Gene ID	2936
mRNA Refseq	NM_000637
Protein Refseq	NP_000628
Uniprot ID	P00390
Chromosome Location	8p21.1
Pathway	Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem;
Function	NADP binding; electron carrier activity; flavin adenine dinucleotide binding; glutathione binding; glutathione-disulfide reductase activity;