Recombinant Human GRM3 protein, His & GST-tagged

• Cat.No.: GRM3-1941H
• Product Overview: Recombinant Human GRM3 aa. (Asp568~Phe819 (Accession# Q14832)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Asp568~Phe819
• Description: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 58.2kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: GRM3 glutamate metabotropic receptor 3 [ Homo sapiens (human) ]
• Official Symbol: GRM3
• Synonyms: MYO1E; myosin IE; FSGS6; MYO1C; HuncM-IC; unconventional myosin-Ie; MYO1E variant protein; myosin-IC; unconventional myosin 1E
• Gene ID: 2913
• mRNA Refseq: NM_000840.2
• Protein Refseq: NP_000831.2
• UniProt ID: Q14832