Recombinant Human GPR161 Protein (1-28 aa), GST-tagged

• Cat.No.: GPR161-1201H
• Product Overview: Recombinant Human GPR161 Protein (1-28 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Partial.

Specification

• Description: Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is roved from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 30.0 kDa
• Protein length: 1-28 aa
• AA Sequence: MSLNSSLSCRKELSNLTEEEGGEGGVII
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: GPR161 G protein-coupled receptor 161 [ Homo sapiens ]
• Official Symbol: GPR161
• Synonyms: GPR161; RE2; FLJ33952
• Gene ID: 23432
• mRNA Refseq: NM_153832
• Protein Refseq: NP_722561
• MIM: 612250
• UniProt ID: Q8N6U8