Recombinant Human GPC1 protein, His-tagged

• Cat.No.: GPC1-5761H
• Product Overview: Recombinant Human GPC1 aa. (Leu114~Lys277) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu114~Lys277
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 8.6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GPC1 glypican 1 [ Homo sapiens (human) ]
• Official Symbol: GPC1
• Synonyms: GPC1; glypican 1; glypican; glypican-1; glypican proteoglycan 1
• Gene ID: 2817
• mRNA Refseq: NM_002081.2
• Protein Refseq: NP_002072.2
• UniProt ID: P35052