Recombinant Human GFRA1 protein, His-tagged

• Cat.No.: GFRA1-1854H
• Product Overview: Recombinant Human GFRA1 aa. (Leu239~Gln350 (Accession # P56159)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Leu239~Gln350
• Description: This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
• Form: Freeze-dried powder
• Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 5.5

Gene Information

• Gene Name: GFRA1 GDNF family receptor alpha 1 [ Homo sapiens (human) ]
• Official Symbol: GFRA1
• Synonyms: GFRA1; GDNF family receptor alpha 1; GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1; GDNF family receptor alpha-1; GDNFR-alpha-1; GPI-linked anchor protein; Glial cell line-derived neurotrophic factor receptor alpha; PI-linked cell-surface accessory protein; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1
• Gene ID: 2674
• mRNA Refseq: NM_001145453.2
• Protein Refseq: NP_001138925.1
• UniProt ID: P56159