Active Recombinant Human GFRA1 protein, His-tagged

• Cat.No.: GFRA1-2168H
• Product Overview: Recombinant Human GFRA1 protein(NP_665736.1)(Met1-Ser424), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser424
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized Recombinant Human GDNF Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human GFRA1 / GFRα1 / GDNFRA Protein (His Tag), the EC50 is 15-48 ng/mL.
• Molecular Mass: The recombinant human GFRα1 consists of 411 amino acids after removal of the signal peptide and predicts a molecular mass of 46 kDa. As a result of glycosylation, the rhGFRα1 migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GFRA1 GDNF family receptor alpha 1 [ Homo sapiens ]
• Official Symbol: GFRA1
• Synonyms: GFRA1; GDNF family receptor alpha 1; GDNFRA; GDNF family receptor alpha-1; GDNFR; GFR ALPHA 1; RET1L; RETL1; TRNR1; RET ligand 1; GDNFR-alpha-1; GDNF receptor alpha-1; GPI-linked anchor protein; PI-linked cell-surface accessory protein; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; Glial cell line-derived neurotrophic factor receptor alpha; GFR-ALPHA-1; FLJ10561; FLJ31546; MGC23045; DKFZp313E1012; DKFZp686J0156
• Gene ID: 2674
• mRNA Refseq: NM_001145453
• Protein Refseq: NP_001138925
• MIM: 601496
• UniProt ID: P56159