Recombinant Rat GFRA1 protein(Met1-Leu445), hFc-tagged

• Cat.No.: GFRA1-2345R
• Product Overview: Recombinant Rat GFRA1 (Q62997) (Met1-Leu445) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Leu445
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized rat His-GDNF (78-211) at 10 μg/ml (100 μl/well) can bind rat GFRA1-Fc, The EC50 of rat GFRA1-Fc is 20-46.6 ng/ml.
• Molecular Mass: The recombinant rat GFRA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 662 amino acids and has a predicted molecular mass of 73.8 kDa. The apparent molecular mass of the protein is approximately 85 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Gfra1 GDNF family receptor alpha 1 [ Rattus norvegicus ]
• Official Symbol: GFRA1
• Synonyms: GFRA1; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNFR-alpha; GFR-alpha-1; RET ligand 1; GDNFR-alpha-1; GDNF receptor alpha; GDNF receptor alpha-1; TGF-beta-related neurotrophic factor receptor 1; Glial cell line-derived neurotrophic factor receptor alpha; glial cell line derived neurotrophic factor family receptor alpha 1
• Gene ID: 25454
• mRNA Refseq: NM_012959
• Protein Refseq: NP_037091