Recombinant Human GFM2 protein, His & T7-tagged

• Cat.No.: GFM2-1731H
• Product Overview: Recombinant Human GFM2 aa. (Asn570~Thr779 (Accession # Q969S9)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors, which is a GTPase that plays a role at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA . Its role in the regulation of normal mitochondrial function and in disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.6kDa
• Protein length: Asn570~Thr779
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 5.6

Gene Information

• Gene Name: GFM2 G elongation factor mitochondrial 2 [ Homo sapiens (human) ]
• Official Symbol: GFM2
• Synonyms: GFM2; G elongation factor mitochondrial 2; EFG2; RRF2; MRRF2; hEFG2; MST027; RRF2mt; EF-G2mt; MSTP027; mEF-G 2; ribosome-releasing factor 2, mitochondrial; mitochondrial elongation factor G2; mitochondrial ribosome recycling factor 2
• Gene ID: 84340
• mRNA Refseq: NM_032380.4
• Protein Refseq: NP_115756.2
• UniProt ID: Q969S9