Recombinant Human GFM1 protein, His & T7-tagged

• Cat.No.: GFM1-7826H
• Product Overview: Recombinant Human GFM1 aa. (Met1~Val158 (Accession # Q96RP9)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.9kDa.
• Protein length: Met1~Val158
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: GFM1 G elongation factor mitochondrial 1 [ Homo sapiens (human) ]
• Official Symbol: GFM1
• Synonyms: ARG2; arginase-2, mitochondrial; arginase, type II; type II arginase
• Gene ID: 85476
• mRNA Refseq: NM_001308164.1
• Protein Refseq: NP_001295093.1
• UniProt ID: Q96RP9