Recombinant Human general transcription factor IIIC, polypeptide 6, alpha 35kDa, His-tagged

• Cat.No.: GTF3C6-662H
• Product Overview: GTF3C6, 1-213aa Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-213 a.a.
• Description: RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcription factors (GTFs). GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins (e.g., GTF3C1, MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA (MIM 180420), tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Recombinant human GTF3C6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In PBS buffer (pH 7.4) containing 10% glycerol, 1mM DTT
• Molecular Mass: 26.4kDa (236aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAAADE RSPEDGEDEE EEEQLVLVEL SGIIDSDFLS KCENKCKVLG IDTERPILQV DSCVFAGEYE DTLGTCVIFE ENVEHADTEG NNKTVLKYKC HTMKKLSMTR TLLTEKKEGE ENIGGVEWLQ IKDNDFSYRP NMICNFLHEN EDEEVVASAP DKSLELEEEE IQMNDSSNLS CEQEKPMHLE IEDSGPLIDI PSETEGSVFM ETQMLP
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by BRADFORD assay)

Gene Information

• Gene Name: GTF3C6 general transcription factor IIIC, polypeptide 6, alpha 35kDa [ Homo sapiens ]
• Official Symbol: GTF3C6
• Synonyms: GTF3C6; general transcription factor IIIC, polypeptide 6, alpha 35kDa; C6orf51, chromosome 6 open reading frame 51; general transcription factor 3C polypeptide 6; bA397G5.3; TFIIIC35; TFIIIC 35 kDa subunit; transcription factor IIIC 35kDa; transcription factor IIIC subunit 6; transcription factor IIIC 35 kDa subunit; C6orf51
• Gene ID: 112495
• mRNA Refseq: NM_138408
• Protein Refseq: NP_612417
• MIM: 611784
• UniProt ID: Q969F1
• Chromosome Location: 6q21
• Function: contributes_to DNA binding; protein binding