Recombinant Human GDNF Protein

• Cat.No.: GDNF-18H
• Product Overview: Recombinant human GDNF protein without tag was expressed in E. coli.
  Animal-free (AOF) and carrier protein-free.
  Mass spectrometry: single species with expected mass.
  Recovery from stock vial: >95%

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing.
• Molecular Mass: 15.1 kDa monomer, 30.1 kDa dimer
• Endotoxin: <0.005 EU/μg protein (below level of detection)
• Applications: Differentiation of midbrain dopaminergic neurons
• Storage: Resuspend in water at >100 μg/mL, prepare single use aliquots, add carrier protein if desired and store frozen at -20 or -80 centigrade
• Storage Buffer: Lyophilized from acetonitrile, TFA

Gene Information

• Gene Name: GDNF glial cell derived neurotrophic factor [ Homo sapiens (human) ]
• Official Symbol: GDNF
• Synonyms: GDNF; glial cell derived neurotrophic factor; ATF; ATF1; ATF2; HSCR3; HFB1-GDNF; glial cell line-derived neurotrophic factor; astrocyte-derived trophic factor
• Gene ID: 2668
• mRNA Refseq: NM_000514
• Protein Refseq: NP_000505
• MIM: 600837
• UniProt ID: P39905