Recombinant Mouse Gdnf Protein, His-tagged

• Cat.No.: Gdnf-1574M
• Product Overview: Recombinant Mouse Gdnf Protein (Asp79-Leu217) with N-His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Asp79-Leu217
• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Homozygous knockout mice for this gene exhibit defects in kidney development and neonatal death. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.9 kDa
  Accurate Molecular Mass: 19 kDa
• Purity: > 97%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gdnf glial cell line derived neurotrophic factor [ Mus musculus (house mouse) ]
• Official Symbol: Gdnf
• Synonyms: GDNF; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; mGDNF; neurotrophic factor; astrocyte-derived trophic factor; AI385739
• Gene ID: 14573
• mRNA Refseq: NM_010275
• Protein Refseq: NP_034405
• UniProt ID: P48540