Recombinant Human GDF15 Protein

• Cat.No.: GDF15-17H
• Product Overview: Recombinant human GDF-15 protein without tag was expressed in E. coli.
  Carrier protein-free.
  Mass spectrometry: single species with expected mass.
  Analytical reversed-phase: single sharp peak.
  Recovery from stock vial: >95%

Specification

• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress.
• Source: E. coli
• Species: Human
• Molecular Mass: 25 kDa (dimer). Mature active protein is a disulfide-linked dimer.
• Endotoxin: <0.005 EU/μg protein (below level of detection)
• Applications: Biomarker for cellular stress; In vivo metabolic studies in mice (using human GDF15)
• Storage: Resuspend in 10mM HCl at >100 μg/mL, prepare single use aliquots, add carrier protein if desired and store frozen at -20 or -80 centigrade
• Storage Buffer: Lyophilized from acetonitrile, TFA

Gene Information

• Gene Name: GDF15 growth differentiation factor 15 [ Homo sapiens (human) ]
• Official Symbol: GDF15
• Synonyms: GDF15; growth differentiation factor 15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; growth/differentiation factor 15; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PTGF-beta; macrophage inhibitory cytokine 1; non-steroidal anti-inflammatory drug-activated gene-1; placental TGF-beta; placental bone morphogenetic protein; prostate differentiation factor
• Gene ID: 9518
• mRNA Refseq: NM_004864
• Protein Refseq: NP_004855
• MIM: 605312
• UniProt ID: Q99988