Recombinant Human GATA3 293 Cell Lysate

• Cat.No.: GATA3-6011HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for GATA binding protein 3 (GATA3), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC201618, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: E, IP, WB. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GATA3 GATA binding protein 3 [ Homo sapiens ]
• Official Symbol: GATA3
• Synonyms: GATA3; GATA binding protein 3; trans-acting T-cell-specific transcription factor GATA-3; HDR; GATA-binding factor 3; HDRS; MGC2346; MGC5199; MGC5445
• Gene ID: 2625
• mRNA Refseq: NM_002051
• Protein Refseq: NP_002042
• MIM: 131320
• UniProt ID: P23771
• Chromosome Location: 10p15
• Pathway: Adipogenesis, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Factors involved in megakaryocyte development and platelet production, organism-specific biosystem; Glucocorticoid receptor regulatory network, organism-specific biosystem; Hemostasis, organism-specific biosystem; IL27-mediated signaling events, organism-specific biosystem
• Function: DNA binding; E-box binding; HMG box domain binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; core promoter proximal region sequence-specific DNA binding; core promoter sequence-specific DNA binding; metal ion binding; nucleic acid binding transcription factor activity; nucleic acid binding transcription factor activity; protein binding; sequence-specific DNA binding transcription factor activity; transcription coactivator activity; transcription factor binding; transcription regulatory region DNA binding; transcription regulatory region sequence-specific DNA binding; zinc ion binding