Recombinant Chicken GAPDH Protein (2-333 aa), His-tagged

• Cat.No.: GAPDH-517C
• Product Overview: Recombinant Chicken GAPDH Protein (2-333 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.

Specification

• Description: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assbly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins.
• Source: E. coli
• Species: Chicken
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 39.6 kDa
• Protein length: 2-333 aa
• AA Sequence: VKVGVNGFGRIGRLVTRAAVLSGKV QVVAINDPFIDLNYMVYMFKYDSTH GHFKGTVKAENGKLVINGHAITIFQ ERDPSNIKWADAGAEYVVESTGVFT TMEKAGAHLKGGAKRVIISAPSADA PMFVMGVNHEKYDKSLKIVSNASCT TNCLAPLAKVIHDNFGIVEGLMTTV HAITATQKTVDGPSGKLWRDGRGAA QNIIPASTGAAKAVGKVIPELNGKL TGMAFRVPTPNVSVVDLTCRLEKPA KYDDIKRVVKAAADGPLKGILGYTE DQVVSCDFNGDSHSSTFDAGAGIAL NDHFVKLVSWYDNEFGYSNRVVDLM VHMASKE
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Gallus gallus (chicken) ]
• Official Symbol: GAPDH
• Synonyms: KNC-NDS6
• Gene ID: 374193
• mRNA Refseq: NM_204305
• Protein Refseq: NP_989636
• UniProt ID: P00356

Reviews

07/14/2022

Customer service was patient, prompt and accurate in answering my queries.

11/26/2021

High protein concentration, good quality, worth buying!

01/02/2021

The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

Q&As

Is GAPDH expression stable across tissues?

GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

What diseases has GAPDH been closely associated with the study of?

GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

What is the relationship between GAPDH and HDAC1?

GAPDH binds to HDAC1.

What are the biological functions of GAPDH?

GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

What are the effects of affecting protein expression of GAPDH?

Interference with GAPDH protein expression results in telomere shortening.