Recombinant Human GABPA 293 Cell Lysate

• Cat.No.: GABPA-6071HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for GA binding protein transcription factor, alpha subunit 60kDa (GABPA) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GABPA GA binding protein transcription factor, alpha subunit 60kDa [ Homo sapiens ]
• Official Symbol: GABPA
• Synonyms: GABPA; GA binding protein transcription factor, alpha subunit 60kDa; GA binding protein transcription factor, alpha subunit (60kD); GA-binding protein alpha chain; E4TF1 60; E4TF1A; human nuclear respiratory factor 2 subunit alpha; NFT2; NRF2; NRF2A; nuclear respiratory factor 2 alpha subunit; GABP subunit alpha; transcription factor E4TF1-60; nuclear respiratory factor 2 subunit alpha; human nuclear respiratory factor-2 subunit alpha; GA binding protein transcription factor alpha subunit 60kDa; E4TF1-60; RCH04A07
• Gene ID: 2551
• mRNA Refseq: NM_001197297
• Protein Refseq: NP_001184226
• MIM: 600609
• UniProt ID: Q06546
• Chromosome Location: 21q21-q22.1
• Pathway: Energy Metabolism, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem
• Function: DNA binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; transcription coactivator activity; transcription regulatory region DNA binding