Recombinant Human full length p300 protein, FLAG-tagged

• Cat.No.: p300-2822H
• Product Overview: Recombinant Human full length p300 protein(accession number NP_001420.2) was expressed in Insect Cell with an N-terminal FLAG tag.

Specification

• Source: Insect Cell
• Species: Human
• Tag: Flag
• Protein length: Full Length
• Form: Supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP
• Bio-activity: 0.5 μg of Histone H4 (Cat. No. 31493) were incubated with 0 μg, 0.25 μg or 0.5 μg of p300 protein, respectively in a 30 μl reaction system including 50 mM Tris pH 8.0, 1 mM TCEP, 0.1 mM EDTA, 50 ng/μl BSA and 20 μM acetyl-CoA for 30 minutes at 30°C.
• Molecular Mass: 265.4 kDa
• Purity: >75%
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: p300