Recombinant Human fucosidase, alpha-L- 2, plasma, His-tagged

• Cat.No.: FUCA2-679H
• Product Overview: FUCA2, 29-467aa Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 29-467 a.a.
• Description: FUCA2 is a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. This protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. FUCA2 is essential for Helicobacter pylori adhesion to human gastric cancer cells. Recombinant human FUCA2 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid, In 20mM phosphate (pH8.0) containing 10% glycerol
• Molecular Mass: 53.3 kDa (462aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHSATRFD PTWESLDARQ LPAWFDQAKF GIFIHWGVFS VPSFGSEWFW WYWQKEKIPK YVEFMKDNYP PSFKYEDFGP LFTAKFFNAN QWADIFQASG AKYIVLTSKH HEGFTLWGSE YSWNWNAIDE GPKRDIVKEL EVAIRNRTDL RFGLYYSLFE WFHPLFLEDE SSSFHKRQFP VSKTLPELYE LVNNYQPEVL WSDGDGGAPD QYWNSTGFLA WLYNESPVRG TVVTNDRWGA GSICKHGGFY TCSDRYNPGH LLPHKWENCM TIDKLSWGYR REAGISDYLT IEELVKQLVE TVSCGGNLLM NIGPTLDGTI SVVFEERLRQ MGSWLKVNGE AIYETHTWRS QNDTVTPDVW YTSKPKEKLV YAIFLKWPTS GQLFLGHPKA ILGATEVKLL GHGQPLNWIS LEQNGIMVEL PQLTIHQMPC KWGWALALTN VI
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: FUCA2 fucosidase, alpha-L- 2, plasma [ Homo sapiens ]
• Official Symbol: FUCA2
• Synonyms: FUCA2; fucosidase, alpha-L- 2, plasma; plasma alpha-L-fucosidase; dJ20N2.5; MGC1314; alpha-L-fucosidase 2; alpha-L-fucoside fucohydrolase 2; RP1-20N2.5
• Gene ID: 2519
• mRNA Refseq: NM_032020
• Protein Refseq: NP_114409
• MIM: 136820
• UniProt ID: Q9BTY2
• Chromosome Location: 6q24
• Pathway: Other glycan degradation, organism-specific biosystem; Other glycan degradation, conserved biosystem
• Function: alpha-L-fucosidase activity; catalytic activity; cation binding; hydrolase activity, acting on glycosyl bonds