Recombinant Human FTL 293 Cell Lysate

• Cat.No.: FTL-6126HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for ferritin, light polypeptide (FTL) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: FTL ferritin, light polypeptide [ Homo sapiens ]
• Official Symbol: FTL
• Synonyms: FTL; ferritin, light polypeptide; ferritin light chain; ferritin L subunit; ferritin L chain; ferritin light polypeptide like 3; L apoferritin; MGC71996; ferritin L-chain; ferritin light polypeptide-like 3; NBIA3
• Gene ID: 2512
• mRNA Refseq: NM_000146
• Protein Refseq: NP_000137
• MIM: 134790
• UniProt ID: P02792
• Chromosome Location: 19q13.33
• Pathway: Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Membrane Trafficking, organism-specific biosystem; Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem; Porphyrin and chlorophyll metabolism, organism-specific biosystem
• Function: ferric iron binding; ferroxidase activity; identical protein binding; iron ion binding; oxidoreductase activity