Recombinant Human Follistatin

• Cat.No.: FST-1617H
• Product Overview: Recombinant Human follistatinexpressed inSf21 cells using a baculovirus expression system. And A DNA sequence encoding amino acid residues Gly 30 to Asp 329 of the human Follistatin precursor was fused to the signal peptide of human CD33, inserted into a suitable expression vector.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Non
• Description: Follistatin (FST) is a glycosylated single-chain protein. It specifically inhibits the secretion of FSH from the pituitary. Follistatin/activin-binding protein binds to heparan sulfate side chains of proteoglycans on the granulosa cell surface to regulate the various actions of activin. Follistatin regulates a variety of biological functions, including cell proliferation, differentiation, and apoptosis. Follistatin plays a critical role in the production of multiple-organ metastasis, predominantly by inhibiting the angiogenesis.
• Molecular Weight: The predicted molecular weight of Recombinant Human FS is 31 kDa. However, the actual molecular weight as observed by migration on SDS Page is 40 kDa.
• State Of Matter: Lyophilized.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human FS was determined by its ability to neutralize Activin-induced bioactivity on K562 cells (erythroid differentiation). The expected ED50 for this effect is typically 0.1 - 0.4 μg/mL in the presence of 7.5 ng/mL of rhActivin A.

Gene Information

• Gene Name: FST follistatin [ Homo sapiens ]
• Synonyms: EFNA5; ephrin-A5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7 ; eph-related receptor tyrosine kinase ligand 7; LERK-7; AL-1; LERK-7; ephrin-A5
• Gene ID: 10468
• mRNA Refseq: NM_006350
• Protein Refseq: NP_006341
• MIM: 136470
• UniProt ID: P19883
• Chromosome Location: 5q11.2
• Pathway: TGF-beta signaling pathway
• Function: heparan sulfate proteoglycan binding; protein binding; signal transducer activity