Recombinant Human FN1 protein, His-tagged

• Cat.No.: FN1-759H
• Product Overview: Recombinant Human FN1 aa. (Ala2206~Asp2337 (Accession # P02751)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.1kDa
• Protein length: Ala2206~Asp2337
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 5.3.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: FN1 fibronectin 1 [ Homo sapiens (human) ]
• Official Symbol: FN1
• Synonyms: FN1; fibronectin 1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; fibronectin; cold-insoluble globulin; migration-stimulating factor
• Gene ID: 2335
• mRNA Refseq: NM_001306129.1
• Protein Refseq: NP_001293058.1
• UniProt ID: P02751