Recombinant Human Fibroblast Growth Factor 20

• Cat.No.: FGF20-178H
• Product Overview: This recombinant Human FGF-20 is a 23.2 kDa protein containing 209 amino acid residues and it was expressed inE.Coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Fibroblast Growth Factor-20 (FGF-20). The FGF family plays important roles in morphogenesis, cellular differentiation, angiogenesis, tissue remodeling, inflammation and oncogenesis. Fibroblast Growth Factor-20 (FGF-20) is a newly discovered neurotrophic factor belonging to the FGF family. FGF-20 is most similar to FGF-9 and FGF-16.
• Supplied: Sterile filtered and the the protein was lyophilized from 20mM Tris, pH7.6 & 1.5M NaCl (no additives).
• Purity: >97% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-20.
• Reconstitution: The lyophilized FGF-20 should be reconstituted in 20mM TRIS, pH7.6 or DI water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions and stored at 4°Cfor up to 1 week or stored at -20℃ for future use.
• Note: If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration greater than 10ug/ml.
• Reactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 10 ng/ml, corresponding to a specific activity of > 1 x 10⁵units/mg.
• Amino Acid Sequence: PLAEVGGFLG GLEGLGQQVG SHFLLPPAGE RPPLLGERRS AAERSARGGP GAAQLAHLHG ILRRRQLYCR TGFHLQILPD GSVQGTRQDH SLFGILEFIS VAVGLVSIRG VDSGLYLGMN DKGELYGSEK LTSECIFREQ FEENWYNTYS SNIYKHGDTG RRYFVALNKD GTPRDGARSK RHQKFTHFLP RPVDPERVPE LYKDLLMYT VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK
• Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted human FGF-20 should be stored in working aliquots at -20°C.

Gene Information

• Gene Name: FGF20 fibroblast growth factor 20 [ Homo sapiens ]
• Synonyms: FGF20; fibroblast growth factor 20; FGF-20
• Gene ID: 26281
• mRNA Refseq: NM_019851
• Protein Refseq: NP_062825
• MIM: 605558
• UniProt ID: Q9NP95
• Chromosome Location: 8p22
• Pathway: MAPK signaling pathway; Melanoma; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
• Function: growth factor activity