Recombinant Human FGL1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: FGL1-3121H
• Product Overview: Biotinylated Recombinant Human FGL1 protein(Q08830)(Asp64-Asn305), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Asp64-Asn305
• Tag: N-His-Avi
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human FGL1, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.9 kDa same as Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FGL1 fibrinogen-like 1 [ Homo sapiens ]
• Official Symbol: FGL1
• Synonyms: FGL1; fibrinogen-like 1; fibrinogen-like protein 1; HFREP 1; hepassocin; liver fibrinogen-related protein 1; hepatocellular carcinoma-related sequence; hepatocyte-derived fibrinogen-related protein 1; HFREP1; HP-041; LFIRE1; LFIRE-1; MGC12455
• Gene ID: 2267
• mRNA Refseq: NM_004467
• Protein Refseq: NP_004458
• MIM: 605776
• UniProt ID: Q08830