Active Recombinant Human FGL1 Protein, HIgG1 Fc-Avi-tagged, Biotinylated

• Cat.No.: FGL1-119HB
• Product Overview: Biotinylated Recombinant Human FGL1 Protein (Leu23-Ile312) is produced by HEK293 Cells expression system. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Leu23-Ile312
• Description: Fibrinogen-like protein 1(FGL1) is also known as HP-041, Hepassocin, HFREP-1, LFIRE-1. The protective effect of fibrinogen-like protein 1 (FGL1) in liver injury has previously been reported. However, studies have shown that FGL1 may be a predictor of GC patients and a target for GC therapy. Immunocytochemical studies revealed that fgl1 selectively binds to defective spermatozoa in the cauda epididymidis. Northern blot analysis and in situ hybridization demonstrated the high expression of fgl1 in the principal cells of the proximal cauda epididymidis. Immunofluorescence analysis using mouse fibrotic lung tissues suggested that fibrotic regions showed increased expressions of Gtse1 and Fgl1, Gtse1 and Fgl1 are suggested to be novel targets for radiation-induced lung fibrosis.
• Predicted N Terminal: Leu 23
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human LAG-3, Mouse IgG2a Fc tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human FGL1, Fc,Avitag with a linear range of 0.156-1.25 μg/mL.
• Molecular Mass: The protein has a calculated MW of 62.3 kDa. The protein migrates as 65 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg by the LAL method.
• Purity: > 95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: FGL1 fibrinogen-like 1 [ Homo sapiens ]
• Official Symbol: FGL1
• Synonyms: FGL1; fibrinogen-like 1; HFREP 1; hepassocin; HFREP1; HP-041; LFIRE1; LFIRE-1; MGC12455
• Gene ID: 2267
• mRNA Refseq: NM_004467
• Protein Refseq: NP_004458
• MIM: 605776
• UniProt ID: Q08830