Recombinant Human FGF21, His-tagged

• Cat.No.: FGF21-104H
• Product Overview: Recombinant Human Fibroblast Growth Factor-21 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and an N-terminal Methionin (bold), having a molecular weight of 19.7 kDa (calculated). The amino acid sequence of the recombinant human FGF21 is 100% homologous to the amino acid sequence of the human FGF21 without signal sequence and contains 14 a. His-tag at N-terminal.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: FGF-21 is a unique member of FGF super family that is reported to be primarily produced by the liver. FGF-21 has recently been shown to have beneficial effects on a variety of metabolic parameters, including glucose and lipid metabolism and insulin sensitivity in animal models and is thought to exert potent antidiabetic and lipid-lowering agent of obesity and type 2 diabetes mellitus. FGF-21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF-21 and activate downstream signaling pathways.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
• Molecular Weight: 21.6 kDa (202aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
• Biological Activity: The ED50 for this effect is 1.4 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell.
• Activity Assay: 1. Cell Line: NIH-3T3 (Mus mosculus/ embryo) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay Medium: serum free RPMI 1640 4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul) 5. Serum Free Starvation: 24hr with RPMI1640 6. Incubation Time: 24 hr (after sample treatment) 7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution)8. Detection method: BrdU assay
• Storage: Can be stored at +4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70℃. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: FGF21 fibroblast growth factor 21 [ Homo sapiens ]
• Synonyms: FGF21; fibroblast growth factor 21; FGF-21
• Gene ID: 26291
• mRNA Refseq: NM_019113
• Protein Refseq: NP_061986
• UniProt ID: Q9NSA1
• Chromosome Location: 19q13.1-qter
• MIM: 609436
• Function: growth factor activity; protein binding