Recombinant Human FFAR1 293 Cell Lysate

• Cat.No.: FFAR1-6257HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for free fatty acid receptor 1 (FFAR1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: FFAR1 free fatty acid receptor 1 [ Homo sapiens ]
• Official Symbol: FFAR1
• Synonyms: FFAR1; free fatty acid receptor 1; G protein coupled receptor 40 , GPR40; FFA1R; G protein-coupled receptor 40; G-protein coupled receptor 40; GPR40; GPCR40
• Gene ID: 2864
• mRNA Refseq: NM_005303
• Protein Refseq: NP_005294
• MIM: 603820
• UniProt ID: O14842
• Chromosome Location: 19q13.1
• Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Free fatty acid receptors, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem
• Function: G-protein coupled receptor activity; fatty acid binding; guanyl-nucleotide exchange factor activity; receptor activity; signal transducer activity