Recombinant Human Fc Fragment Of IgG, High Affinity Ia, Receptor (CD64), AVI-tagged

• Cat.No.: FCGR1A-3818H
• Product Overview: A DNA sequence encoding the extracellular domain of human CD64 ( NP_000557.1 ) ( Met 1 - Thr 287 ) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus The expressed protein was biotinylated in vivo by the Biotin-Protein ligase ( BirA enzyme ) which is co-expressed.

Specification

• Cat. No.: FCGR1A-3818H
• Description: High affinity immunoglobulin gamma Fc receptor IA , also known as IgG Fc receptor IA (FCGR1A) and CD64, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity. CD64 belongs to the immunoglobulin superfamily. It is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail.
• Source: Human
• Host: Human Cells
• Form: Lyophilized from sterile PBS , pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
• Predicted N terminal: Gln 16
• Molecular Mass: The secreted recombinant human CD64 consists of 307 amino acids and has a predicted molecular mass of 34.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD64 is approximately 50-55 kDa due to glycosylation.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Protein length: 1-287 a.a.

Gene Information

• Gene Name: FCGR1A Fc fragment of IgG, high affinity Ia, receptor (CD64) [ Homo sapiens ]
• Official Symbol: FCGR1A
• Synonyms: FCGR1A; Fc fragment of IgG, high affinity Ia, receptor (CD64); CD64; FCRI; CD64A; IGFR1; FLJ18345; FCGR1A; high affinity immunoglobulin gamma Fc receptor I; fcgammaRIa; Fc-gamma RI; fc-gamma RIA; Fc gamma receptor; IgG Fc receptor I; Fc-gamma receptor I A1; Fc fragment of IgG, high affinity Ia, receptor for (CD64); FcRI; CD64 antigen
• Gene ID: 2209
• mRNA Refseq: NM_000566
• Protein Refseq: NP_000557
• MIM: 146760
• UniProt ID: P12314
• Chromosome Location: 1q21.2-q21.3
• Pathway: Fc gamma R-mediated phagocytosis; Hematopoietic cell lineage; Leishmania infection; Systemic lupus erythematosus
• Function: receptor signaling protein activity; IgG binding; protein binding; receptor activity