Recombinant Human FASN, GST-tagged

• Cat.No.: FASN-21H
• Product Overview: Recombinant Human FASN(2378 a.a. - 2477 a.a.), fused with GST-tag at N-terminal, was expressed in wheat germ.

Specification

• Description: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
• Source: Wheat germ
• Species: Human
• Tag: GST
• Molecular Mass: 36.74 kDa
• AA Sequence: MEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGG AYGEDLGADYNLSQVCDGKVSVHVI
• Applications: ELISA; WB-Re; AP; Array
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: FASN fatty acid synthase [ Homo sapiens ]
• Official Symbol: FASN
• Synonyms: FASN; fatty acid synthase; FAS; SDR27X1; short chain dehydrogenase/reductase family 27X; member 1
• Gene ID: 2194
• mRNA Refseq: NM_004104
• Protein Refseq: NP_004095
• MIM: 600212
• UniProt ID: P49327
• Chromosome Location: 17q25
• Pathway: AMPK signaling; Activation of gene expression by SREBF (SREBP); Defective AMN causes hereditary megaloblastic anemia 1
• Function: 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; [acyl-carrier-protein] S-acetyltransferase activity