Recombinant human FAM84B, His-tagged

• Cat.No.: FAM84B-2442H
• Product Overview: Recombinant human FAM84B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Description: FAM84B, as known as Family with sequence similarity 84, member B, coprecipitated with a downstream effector of RAS, CRAF. Binding of FAM83B with CRAF disrupted CRAF/14-3-3 interactions and increased CRAF membrane localization, resulting in elevated MAPK and mammalian target of rapamycin (mTOR) signaling. It is an oncogene and potentially represents a new target for therapeutic intervention.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.15M NaCl,1mM DTT, 0.1mM PMSF
• Molecular Mass: 36.9 kDa(333aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGNQVEK LTHLSYKEVP TADPTGVDRD DGPRIGVSYI FSNDDEDVEP QPPPQGPDGG GLPDGGDGPPPPQPQPYDPR LHEVECSVFY RDECIYQKSF APGSAALSTY TPENLLNKCK PGDLVEFVSQ AQYPHWAVYV GNFQVVHLHR LEVINSFLTDASQGRRGRVV NDLYRYKPLS SSAVVRNALA HVGAKERELS WRNSESFAAW CRYGKREFKI GGELRIGKQP YRLQIQLSAQ RSHTLEFQSLEDLIMEKRRN DQIGRAAVLQ ELATHLHPAE PEEGDSNVAR TTPPPGRPPA PSSEEEDGEA VAH
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: FAM84B family with sequence similarity 84, member B [ Homo sapiens ]
• Official Symbol: FAM84B
• Synonyms: FAM84B; family with sequence similarity 84, member B; protein FAM84B; BCMP101; breast cancer membrane associated protein 101; neurological/sensory 2; NSE2; breast cancer membrane protein 101; breast cancer membrane-associated protein 101
• Gene ID: 157638
• mRNA Refseq: NM_174911
• Protein Refseq: NP_777571
• MIM: 609483
• UniProt ID: Q96KN1
• Chromosome Location: 8q24.13
• Function: protein binding