Recombinant Human F9 protein, His-tagged

• Cat.No.: F9-7807H
• Product Overview: Recombinant Human F9 aa. (Asp232~Lys455 (Accession # P00740)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.5kDa
• Protein length: Asp232~Lys455
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.9.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: F9 coagulation factor IX [ Homo sapiens (human) ]
• Official Symbol: F9
• Synonyms: F9; coagulation factor IX; FIX; P19; PTC; HEMB; THPH8; F9 p22; coagulation factor IX; Christmas factor; factor 9; factor IX F9; plasma thromboplastic component; plasma thromboplastin component
• Gene ID: 2158
• mRNA Refseq: NM_000133.3
• Protein Refseq: NP_000124.1
• UniProt ID: P00740