Recombinant Human Erythropoietin

• Cat.No.: EPO-01H
• Product Overview: Recombinant Human EPO is expressed in CHO cells. The mature protein consists of a 165 amino acid polypeptide chain heavily glycosylated at three N-linked and O-linked glycosylation sites yielding a total molecular mass of 35-45kDa. About 40% of the fully glycosylated EPO molecule consists of carbohydrate.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Non
• Description: Erythropoietin (EPO) is a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
• Purity: ≥98% as determined by SDS-PAGE and HPLC analyses
• Formulation: Sterile filtered lyophilized powder.
• Specific Activity: rHuEPO is fully biologically active when compared to standards. Its specific activity is ≥1.2×10⁵IU/mg.
• Endotoxin: Less than 0.1EU/µg determined by LAL test.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuEPO in sterile buffer not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storagee: Lyophilized rHuEPO although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Reconstituted rHuEPO aliquots should be stored at –20°C for maximal stability up to three years. Aliquot to avoid repeated freeze-thaw cycles.
• Publications:
  Dual Delivery of BMP2 and VEGF from a modular polycaprolactone scaffold for the treatment of large bone. (2015)
  Dual Delivery of BMP2 and VEGF from a polycaprolactone/collagen sponge construct to increase bone growth in ectopic sites for flap prefabrication. (2015)
  Dual Delivery of EPO and BMP2 from a Novel Modular Poly-?-Caprolactone Construct to Increase the Bone Formation in Prefabricated Bone Flaps. (2015)
  Oxalic Acid Supported Si–18F-Radiofluorination: One-Step Radiosynthesis of N-Succinimidyl 3-(Di-tert-butyl [18F] fluorosilyl) benzoate ([18F] SiFB) for Protein Labeling (2012)

Gene Information

• Gene Name: EPO
• Synonyms: EP; MGC138142; Epoetin; Erythropoietin Precursor; EPO; erythropoietin
• Gene ID: 2056
• mRNA Refseq: NM_000799
• Protein Refseq: NP_000790
• MIM: 133170
• UniProt ID: P01588
• Chromosome Location: 7q22
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway
• Function: erythropoietin receptor binding IEA ;hormone activity IEA ;protein binding