Active Recombinant Human EPO, HIgG1 Fc-tagged, mutant

• Cat.No.: EPO-002H
• Product Overview: The extracellular domain of human cEPO(NP_000790.2)(Pro30-Arg193) (Arg103Glu) is fused to the N-terminus of the Fc region of a mutant human IgG1 was expressed in CHO cell.

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• Protein Length: 30-193 a.a.
• Description: This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
• Form: Lyophilized from 0.2 μm-filtered solution in PBS.
• Bio-activity: Measured by the dose-dependant stimulation of human megakaryoblastic leukemia cells.
• Molecular Mass: 44KDa (monomer)
• AA Sequence: (Pro30-Arg193) (Arg103Glu)
• Endotoxin: <0.06 eu/μg="" as="" determined="" by="" lal="" test.="">
• Purity: >98%, by SDS-PAGE under reducing conditions
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Reconstitution: Reconstitute at 100 μg/ml in sterile PBS.
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: EPO erythropoietin [ Homo sapiens ]
• Official Symbol: EPO
• Synonyms: EPO; erythropoietin; EP; epoetin; MVCD2; MGC138142
• Gene ID: 2056
• mRNA Refseq: NM_000799
• Protein Refseq: NP_000790
• MIM: 133170
• UniProt ID: P01588
• Chromosome Location: 7q21
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; EPO Receptor Signaling, organism-specific biosystem; EPO signaling pathway, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem
• Function: erythropoietin receptor binding; eukaryotic cell surface binding; hormone activity; protein binding; protein kinase activator activity