Recombinant Human ERN1 Overexpression Lysate(Pro 465-Leu 977)

• Cat.No.: ERN1-001HCL
• Product Overview: Recombinant Human ERN1 Overexpression Lysate(O75460-1)(Pro 465-Leu 977) was expressed in Insect cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect cells
• Tag: GST&His
• Protein Length: 465-977 a.a.
• Form: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Molecular Mass: The recombinant human ERN1/GST chimera consists of 750 amino acids and has a calculated molecular mass of 86 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
• Storage: Store at 4°C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80°C for up to twelve months. Avoid repeated freeze-thaw cycles.
• Reconstitution: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.

Gene Information

• Gene Name: ERN1 endoplasmic reticulum to nucleus signaling 1 [ Homo sapiens (human) ]
• Official Symbol: ERN1
• Synonyms: IRE1; IRE1P; IRE1a; hIRE1p; ERN1
• Gene ID: 2081
• mRNA Refseq: NM_001433
• Protein Refseq: NP_001424
• MIM: 604033
• UniProt ID: O75460