Recombinant Human ERN1 Protein, His-Avi-Tagged

• Cat.No.: ERN1-01H
• Product Overview: Recombinant human ERN1 protein, His-Avi-Tagged was expressed in Sf9 insect cells

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Avi&His
• Description: This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Purity: ≥90%
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 300 mM Imidazole
• Reconstitution: Assay was done in a Kinase buffer using MBP (NEB, 0.1 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
• Storage: At least 6 months at -80°C
• Warning: Avoid freeze/thaw cycles.
• Format: Aqueous buffer solution
• Shipping: -80°C

Gene Information

• Gene Name: ERN1 endoplasmic reticulum to nucleus signaling 1 [ Homo sapiens (human) ]
• Official Symbol: ERN1
• Synonyms: IRE1; IRE1P; IRE1a; hIRE1p
• Gene ID: 2081
• mRNA Refseq: NM_001433.5
• Protein Refseq: NP_001424.3
• UniProt ID: O75460