Recombinant Human Epidermal Growth Factor Receptor, His-tagged

• Cat.No.: EGFR-315H
• Product Overview: EGFR contains C terminal 6 His Tag and has a calculated MW of 69.5 kDa. In DTT-reduced SDS-PAGE, protein migrates as 110-115 kDa.

Specification

• Cat. No.: EGFR-315H
• Description: The epidermal growth factor receptor (EGFR; ErbB 1; HER1 in humans) is the cell surface receptor for members of the The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.[1] The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
• Source: Recombinant human EGFR (rhEGFR) Leu25 - Ser645 (Accession P00533) fused with C terminal 6 His Tag was expressed in human 293 cells (HEK293).
• Species: Human
• Form: Bulk protein in a 0.22 μm filtered solution of PBS, pH7.4 and delivered as lyophilized powder, Normally 2-7% Mannitol and Trehalose before lyophilization are 7% Mannitol and Trehalose before lyophilization are added as protectants. It is recommended to reconstitute the lyophilized rhEGFR in sterile dH2O.
• Purity: >95% purity as determined by SDS-PAGE of reduced rhEGFR.
• Endotoxin: Less than 0.01 ng endotoxin 1 μg rhEGFR.
• Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; EC 2.7.10.1; ERBB; ERBB1; HER1; PIG61; mENA; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog); epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); EC 2.7.10; Proto-oncogene c-ErbB-1; OTTHUMP00000159661; OTTHUMP00000159662; OTTHUMP00000159663; OTTHUMP00000209210; OTTHUMP00000209211
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533
• Chromosome Location: 7p12
• Pathway: Adherens junction; Calcium signaling pathway; Colorectal cancer; Cytokine-cytokine receptor interaction; Dorso-ventral axis formation; Endometrial cancer; Epithelial cell signaling in Helicobacter pylori infection; ErbB signaling pathway; Focal adhesion; Gap junction; Glioma; GnRH signaling pathway; MAPK signaling pathway; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Prostate cancer; Regulation of actin cytoskeleton
• Function: ATP binding; actin filament binding; double-stranded DNA binding; epidermal growth factor receptor activity; identical protein binding.