Recombinant Human EPHB6 protein(Met1-Ser579)

• Cat.No.: EPHB6-698H
• Product Overview: Recombinant Human EphB6 (NP_004436.1) (Met 1-Ser 579) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Non
• Protein length: 1-579 a.a.
• Form: Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human EphB6 at 10 μg/mL (100 μl/well) can bind biotinylated human EFNB2-Fch with a linear range of 1.56-50 ng/mL.
• Molecular Mass: The recombinant mature human EphB6 consists of 569 amino acids and has a calculated molecular mass of 60.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphB6 is approximately 72 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHB6 EPH receptor B6 [ Homo sapiens ]
• Official Symbol: EPHB6
• Synonyms: EPHB6; EPH receptor B6; EphB6; ephrin type-B receptor 6; HEP; tyrosine-protein kinase-defective receptor EPH-6; MGC129910; MGC129911
• Gene ID: 2051
• mRNA Refseq: NM_004445
• Protein Refseq: NP_004436
• MIM: 602757
• UniProt ID: O15197