Recombinant Human EphB6 protein(Met1-Ser579), His-tagged

• Cat.No.: EPHB6-3152H
• Product Overview: Recombinant Human EphB6 (NP_004436.1)(Met 1-Ser 579) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-579 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human EphB6 His at 2 μg/mL (100 μL/well) can bind Human Ephrin B2 His & hFc, the EC50 of Human Ephrin B2 His & hFc is 6-48ng/mL.
• Molecular Mass: The secreted recombinant human EphB6 consists of 574 amino acids and has a calculated molecular mass of 61.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphB6 is approximately 60-70 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHB6 EPH receptor B6 [ Homo sapiens ]
• Official Symbol: EphB6
• Synonyms: EPHB6; EPH receptor B6; EphB6; ephrin type-B receptor 6; HEP; tyrosine-protein kinase-defective receptor EPH-6; MGC129910; MGC129911
• Gene ID: 2051
• mRNA Refseq: NM_004445
• Protein Refseq: NP_004436
• MIM: 602757
• UniProt ID: O15197