Recombinant Human EPHA3 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: EPHA3-8547HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human EPHA3 (Met1-Gln541), fused with a polyhistidine tag at the C-terminus, was produced in Human Cells.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 532
• Form: Lyophilized
• Molecular Mass: The recombinant human EPHA3 consists of 532 amino acids and predicts a molecular mass of 60.3 kDa. It migrates as an approximately 65-70 kDa band in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Glu 21
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA3 EPH receptor A3 [ Homo sapiens ]
• Official Symbol: EPHA3
• Gene ID: 2042
• mRNA Refseq: NM_005233
• Protein Refseq: NP_005224
• MIM: 179611
• UniProt ID: P29320