Active Recombinant Human EPHA3 Protein, GST-tagged

• Cat.No.: EPHA3-3381H
• Product Overview: Human EPHA3 (NP_005224.2, 1 a.a. - 464; 1 a.a. - 216 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
  Substrate: Blk/Lyntide.
  ATP: 100 μM.
• Molecular Mass: 73 kDa
• Purity: 88 % by SDS-PAGE/CBB staining.
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: EPHA3 EPH receptor A3 [ Homo sapiens ]
• Official Symbol: EPHA3
• Synonyms: EPHA3; EPH receptor A3; EphA3 , ETK, ETK1, TYRO4; ephrin type-A receptor 3; HEK; HEK4; EPH-like kinase 4; human embryo kinase 1; eph-like tyrosine kinase 1; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1; EK4; ETK; ETK1; TYRO4
• Gene ID: 2042
• mRNA Refseq: NM_005233
• Protein Refseq: NP_005224
• MIM: 179611
• UniProt ID: P29320