Recombinant Human EPH Receptor B1, GST-His

• Cat.No.: EPHB1-746H
• Product Overview: Recombinant Human EPHB1, Amino acids R565-A984, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 76,906 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 565-984 a.a.
• Description: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: EPHB1, Lot 001, was confirmed as human EPHB1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
• Concentration: 0.500 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 72 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: EPHB1 EPH receptor B1 [ Homo sapiens ]
• Synonyms: EPHB1; EPH receptor B1; ELK; NET; Hek6; EPHT2; FLJ37986; ephrin type-B receptor 1; EK6; EPH-like kinase 6; OTTHUMP00000216708; eph tyrosine kinase 2; soluble EPHB1 variant 1; tyrosine-protein kinase receptor EPH-2; EC 2.7.10.1
• Gene ID: 2047
• mRNA Refseq: NM_004441
• Protein Refseq: NP_004432
• MIM: 600600
• UniProt ID: P54762
• Chromosome Location: 3q21-q23
• Pathway: Axon guidance
• Function: ATP binding; axon guidance receptor activity; ephrin receptor activity; nucleotide binding; protein binding; receptor activity; transferase activity; transmembrane receptor protein tyrosine kinase activity