Recombinant Human EPB42 protein, His & GST-tagged

• Cat.No.: EPB42-1406H
• Product Overview: Recombinant Human EPB42 aa. (Leu475~Ala686 (Accession # P16452) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Erythrocyte membrane protein band 4.2 is an ATP-binding protein which may regulate the association of protein 3 with ankyrin. It probably has a role in erythrocyte shape and mechanical property regulation. Mutations in the EPB42 gene are associated with recessive spherocytic elliptocytosis and recessively transmitted hereditary hemolytic anemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 53.8kDa.
• Protein length: Leu475~Ala686
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 8.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: EPB42 erythrocyte membrane protein band 4.2 [ Homo sapiens (human) ]
• Official Symbol: EPB42
• Synonyms: EPB42; erythrocyte membrane protein band 4.2; PA; SPH5; P4.2; erythrocyte protein 4.2; erythrocyte surface protein band 4.2
• Gene ID: 2038
• mRNA Refseq: NM_000119.2
• Protein Refseq: NP_000110.2
• UniProt ID: P16452