Recombinant Human EIF4A2 protein, His-tagged

• Cat.No.: EIF4A2-47H
• Product Overview: Recombinant Human EIF4A2, fused with N-His tag, was expressed in E. coli.

Specification

• Description: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5"-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
• Source: E.coli
• Species: Human
• Tag: His
• Form: 20mM Tris.Cl, 50mM NaCl, 20% Glycerol, pH8.0.
• Molecular Mass: 48.3kDa(including fusion tag)
• Protein length: 422aa(including fusion tag)
• Applications: WB, ELISA, IP, antibody production, protein array
• Storage: Store at -80°C and avoid repeated freeze-thaw cycles

Gene Information

• Gene Name: EIF4A2 eukaryotic translation initiation factor 4A2 [ Homo sapiens ]
• Official Symbol: EIF4A2
• Synonyms: EIF4A2; BM 010; DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
• Gene ID: 1974
• mRNA Refseq: NM_001967
• Protein Refseq: NP_001958
• MIM: 601102
• UniProt ID: Q14240
• Chromosome Location: Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Deadenylation of mRNA, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem
• Pathway: ATP binding; ATP-dependent helicase activity; RNA binding; helicase activity; hydrolase activity; nucleic acid binding; nucleotide binding