Recombinant Human EGFR Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: EGFR-3803HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human EGFR (NP_005219) extracellular domain (Met 1-Lys 642), fused with a C-terminal polyhistidine tag, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect cells
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant human EGFR consists of 626 amino acids and has a calculated molecular mass of 69.2 kDa. The recombinant protein migrates as an approximately 75 kDa protein in SDS-PAGE under reducing conditions.
• Protein length: 626
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 50 mM NaCl, 3vmM DTT, 0.5 mM TCEP, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533