Active Recombinant Human EGFR, His&Avi-tagged, Biotinylated

• Cat.No.: EGFR-285H
• Product Overview: Recombinant Human EGFR(Leu 25 - Ser 645), fused with His&Avi-tag at C-terminal, Biotinylated, was produced in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Human
• Tag: His&Avi
• Predicted N Terminal: Leu 25
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized CetuxiMab at 2 μg/mL (100 μL/well) can bind Biotinylated rh EGFR, Avi Tagged with a linear range of 0.002-0.2 μg/mL.
• Molecular Mass: Have a calculated MW of 71.2 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 110-115 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated rh EGFR /ErbB1, His.Avi Tag by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Applications: By co-expression with biotin ligase (BirA enzyme), Biotinylated Human EGFR /ErbB1, His.Avi Tag was biotinylated in vivo for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; epidermal growth factor receptor; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533
• Chromosome Location: 7p12
• Pathway: AGE/RAGE pathway, organism-specific biosystem; Adherens junction, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem
• Function: ATP binding; MAP kinase kinase kinase activity; chromatin binding