Recombinant Human EGFR protein, His & S-tagged

• Cat.No.: EGFR-7629H
• Product Overview: Recombinant Human EGFR aa. (Lys713~Asp984 (Accession # P00533)) fused with N-terminal His & S tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
• Source: E. coli
• Species: Human
• Tag: His&S
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 37.5kDa
• Protein length: Lys713~Asp984
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens (human) ]
• Official Symbol: EGFR
• Synonyms: ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; epidermal growth factor receptor
• Gene ID: 1956
• mRNA Refseq: NM_001346897.1
• Protein Refseq: NP_001333826.1
• UniProt ID: P00533