Active Recombinant Mouse EGFR, His tagged

• Cat.No.: EGFR-101M
• Product Overview: Recombinant Mouse EGFR (Q01279) extracellular domain (Met 1-Ser 647), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc, The EC50 of human EGF-Fc is 60-90 ng/mL.
  3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc, The EC50 of mouse EGF-Fc is 70-100 ng/mL.
• Molecular Mass: The secreted recombinant mouse EGFR comprises 634 amino acids and has a calculated molecular mass of 70.8 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR is approximately 100 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met 1-Ser 647
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. >85% as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Egfr epidermal growth factor receptor [ Mus musculus ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; waved 2; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; Wa5; wa2; Erbb; Errp; wa-2; AI552599; 9030024J15Rik
• Gene ID: 13649
• mRNA Refseq: NM_007912
• Protein Refseq: NP_031938