Recombinant Human EDF1, His-tagged

• Cat.No.: EDF1-2774H
• Product Overview: Recombinant human EDF1 protein, fused to His-tag at C-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 17.4 kDa (156aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Endothelial differentiation-related factor 1, also known as EDF1 may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. Also, EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QH SI TKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGLTQKDLATKIN EKP QVIA DYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: EDF1 endothelial differentiation-related factor 1 [ Homo sapiens ]
• Synonyms: endothelial differentiation-related factor 1; MBF1; EDF-1; MGC9058; EDF1; endothelial differentiation-related factor 1; OTTHUMP00000022618; multiprotein bridging factor 1; multiprotein bridging factor-1; multiprotein-bridging factor 1; Multiprotein-bridging factor 1
• Gene ID: 8721
• mRNA Refseq: NM_003792
• Protein Refseq: NP_003783
• MIM: 605107
• UniProt ID: O60869
• Chromosome Location: 9q34.3
• Function: calmodulin binding; NOT histone acetyltransferase activity; NOT methyltransferase activity; protein binding; sequence-specific DNA binding; transcription activator activity; transcription coactivator activity; NOT transcription factor activity