Recombinant Human DR1, His-tagged

• Cat.No.: DR1-2777H
• Product Overview: Recombinant human DR1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography.MW = 21.6 kDa(196aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 100 mM NaCl, 0.1 mM PMSF ,10% Glycerol.
• Purity: > 85% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MASSSGNDDD LTIPRAAINK MIKETLPNVR VAND ARELVV NCCTEFIHLI SSEANEICNK SEKKTISPEHVIQALESLGF GSYISEVKEV LQE CKTVALK RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WL QMQQAAQQ AQLAAASASA SNQAGSSQDE EDDDDI.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DR1 down-regulator of transcription 1, TBP-binding (negative cofactor 2) [ Homo sapiens ]
• Synonyms: DR1; down-regulator of transcription 1, TBP-binding (negative cofactor 2); NC2; NC2-BETA; down-regulator of transcription 1; OTTHUMP00000012557; Protein Dr1; TATA-binding protein-associated phosphoprotein; Negative cofactor 2-beta; NC2-beta
• Gene ID: 1810
• mRNA Refseq: NM_001938
• Protein Refseq: NP_001929
• MIM: 601482
• UniProt ID: Q01658
• Chromosome Location: 1p22.1
• Function: sequence-specific DNA binding; transcription corepressor activity; transcription factor binding