Recombinant Human DNMT3A Protein, GST/StrepII-tagged

• Cat.No.: DNMT3A-01H
• Product Overview: Human recombinant DNMT3a (residues 214-912, C-term) was expressed in Sf9 insect cells with an N-terminal GST-tag and C-terminal StrepII tag. DNA (cytosine-5)-methyltransferase 3a (DNMT3a) is required for de novo DNA methylation and methylates at both CpG and non-CpG sites.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&Strep II
• Protein Length: 214-912
• Description: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
• Molecular Mass: 107 kDa
• Purity: > 80% by SDS-PAGE.
• Usage: DNMT3a (214-912) displays DNA methyltransferase activity at concentrations of 60 nM and above, 120 min. reactions, 30 centigrade, as TCA-precipitated counts in a scintillation/filter plate assay. Reaction conditions are: 50 mM Tris, pH 8, 5 mM EDTA, 10% glycerol, 10 mM BME, 0.05 mM PMSF with 1.5 μM [3H]-SAM and 10 μg/mL Lambda DNA as substrate.
• Concentration: 1.62 μg/μL
• Storage Buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 3 mM DTT, 0.05% Tween 20

Gene Information

• Gene Name: DNMT3A DNA methyltransferase 3 alpha [ Homo sapiens (human) ]
• Official Symbol: DNMT3A
• Synonyms: DNMT3A; DNA methyltransferase 3 alpha; TBRS; HESJAS; DNMT3A2; M.HsaIIIA; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; EC 2.1.1.37
• Gene ID: 1788
• mRNA Refseq: NM_175629
• Protein Refseq: NP_783328
• MIM: 602769
• UniProt ID: Q9Y6K1